Focusing on neuronal electrical activity, synaptic transmission and network function, we combine patch-clamp electrophysiology, voltage imaging, calcium imaging and ion channel screening in cell and acute slice models to support functional assessment for neuroscience research and drug discovery.
Record membrane potential, action potentials and, where applicable, synaptic currents in brain slices, cultured brain neurons, dorsal root ganglion (DRG) sensory neurons and spinal cord slices to analyze excitability and pharmacological responses.
Acute Brain Slice ElectrophysiologyBrain Neuron ElectrophysiologyDRG Sensory Neuron ElectrophysiologySpinal Cord Slice Patch-Clamp RecordingObserve neural activity through voltage and calcium measurements. Analyze membrane potential dynamics, activity frequency, network synchrony and changes following stimulation or drug treatment in the context of the model conditions.
Neuronal Voltage ImagingNeuronal Calcium ImagingUse a stable NaV1.7-expressing cell line and manual patch clamp for candidate compound activity screening, concentration-response analysis and confirmation of activity to support pain-related drug research.
NaV1.7 Patch-Clamp ScreeningCompare neuronal function across models or treatment conditions using passive membrane properties, action potentials and synaptic currents. Brain slice studies can include synaptic plasticity analysis according to the study protocol.
Acute Brain Slice ElectrophysiologyBrain Neuron ElectrophysiologySelect electrophysiological assays at the DRG sensory neuron, spinal cord slice and NaV1.7 target levels to analyze sensory input and candidate compound effects.
DRG Sensory Neuron ElectrophysiologySpinal Cord Slice Patch-Clamp RecordingNaV1.7 Patch-Clamp ScreeningCombine voltage and calcium signals to observe neuronal population activity and changes following drug treatment. Specific endpoints are determined by the model, probe and acquisition conditions.
Neuronal Voltage ImagingNeuronal Calcium ImagingConfirm the cell source, target brain region or slice type and specify the excitability, synaptic function, network activity or target pharmacology endpoints to be evaluated.
Select the recording approach according to sample condition and establish stimulation, dosing, control groups and analysis parameters. Interpret results from each method according to its measurement principle.
Compare key parameters and pharmacological responses according to the experimental plan. Interpret results in the context of model conditions and data quality to support subsequent mechanistic research.
SparkMind (Nanjing) Technology Co., Ltd.
Rooms 1902–1904, 19th Floor, Building A, ZhongDan Eco Life Science Industrial Park
No. 3-1 Xinjinghu Road, Jiangbei New Area, Nanjing, Jiangsu Province, China
info@sparkmindcn.com
+86 25 5827 7019
