Service Positioning

This service is positioned to support mechanistic electrophysiology studies where high-resolution, cell-level electrical recordings are required. Primary cardiomyocytes preserve native ion channel composition and intrinsic electrophysiological properties, enabling precise evaluation of how compounds, genetic modifications, or experimental interventions affect cardiac excitability.

Primary Rodent Cardiomyocytes

Primary cardiomyocytes isolated from rodent hearts provide a well-established experimental system for studying cardiac electrophysiology. These cells maintain native expression of cardiac ion channels and exhibit physiologically relevant action potentials suitable for detailed functional analysis.

The use of primary cardiomyocytes enables direct interrogation of cardiac electrical mechanisms under controlled in vitro conditions, supporting hypothesis-driven research and targeted electrophysiological validation.

Manual Patch Clamp Electrophysiology

Manual patch clamp remains the gold standard for high-fidelity measurement of cardiomyocyte electrical activity. In both voltage-clamp and current-clamp configurations, this technique allows direct recording of ionic currents and action potentials from individual cells.

This approach is particularly suitable for resolving subtle changes in current kinetics, membrane excitability, and action potential morphology that may not be detectable using indirect or higher-throughput methods.

Typical Electrophysiological Readouts

• Action potential waveform and duration parameters
• Resting membrane potential and excitability
• Major cardiac ion currents and their kinetics
• Drug- or intervention-induced changes in electrical behavior
• Cell-to-cell variability within experimental groups

Applications

• Mechanistic studies of cardiac ion channel function
• Functional validation of targets or genetic models
• Detailed electrophysiological characterization of compounds
• Supportive cardiac electrophysiology data for research programs